西西影院,色七七亚州,人人洗人人干,亚洲天堂自拍偷怕

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CCL-171MRC-5 人胚肺細(xì)胞

MRC-5 人胚肺細(xì)胞

簡(jiǎn)要描述:CCL-171 MRC-5 人胚肺細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CCL-171
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:1955

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

CCL-171 MRC-5 人胚肺細(xì)胞

ATCC® Number:  CCL-171™

Designations:  MRC-5

Depositors:   JP Jacobs

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

CCL-171 MRC-5 人胚肺細(xì)胞

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation:  Isolation date: September, 1966

Applications: efficacy testing [92587]

testing [92346] [92389]

transfection host (Roche FuGENE® Transfection Reagents)

viruscide testing [92579] [92582]

Virus Susceptibility: poliovirus 1; herpes simplex; vesicular stomatitis (Indiana)

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11,14

D16S539: 9,11

D5S818: 11,12

D7S820: 10,11

THO1: 8

TPOX: 8

vWA: 15

Cytogenetic Analysis: Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal.

Isoenzymes:  G6PD, B

Age:  14 weeks gestation

Gender:  male

Ethnicity:  Caucasian


Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

CCL-171



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
www.狠狠| 亚洲天堂网av在线| 韩日欧美色| 2022欧美日韩精品| 亚洲动漫精品| 影音先锋东京热二区| 日韩精品熟妇二区三区| 亚洲av杨幂性猛交xxx| AV人摸人人人澡人人超碰妓女| 天天哑州天天色| 日韩不卡网| 欧美精品熟妇在线| 被窝影院| 综合激情网站| 亚洲av天天玩| 成人免费3区| 国产我和子的乱视频| 日韩人妻无码一区| 亚洲一本不卡| 东京热人妻系列| 69av在线观看免费| 污污网站免费在线观看| 91操美女| 东京无码人妻AV| 国产嫖妓一区| 国产亚洲天堂无码| 午夜精品久久久久久久久久久久久| 国产精品va无码一区二区| 久久黄色免费| 高清无码666| 最新在线网址你懂得| 爆乳无码| 精品一区二区不卡无码av| 另类亚洲天堂| www.狠狠热| 欧美精品国产字幕| 日本成年人网站| 国产熟女一区二区三区四区五区 | 无码人妻精品一区二区三区99日韩| 丁香五月天堂| 中国性孕妇孕交tv|