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簡要描述：CRL-5974 SNU-16 人胃癌細胞 腹水，原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件！
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CRL-5974 SNU-16 人胃癌細胞 腹水
ATCC® Number: CRL-5974™ Price: $425.00
Designations: SNU-16
Depositors: J Park
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, multicell aggregates
Organism: Homo sapiens （human）
Morphology: epithelial
Source: Organ: stomach
Disease: gastric carcinoma
Derived from metastatic site: ascites
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-5974 SNU-16 人胃癌細胞 腹水
Isolation: Isolation date: 1987
Receptors: vasoactive intestinal peptide （VIP）, expressed [23078]
Oncogene: myc +; erb B2 +
Antigen Expression: Blood Type A; Rh +
The cells express the surface glycoproteins carcinoembryonic antigen （CEA） and TAG 72.
DNA Profile （STR）: Amelogenin: X
CSF1PO: 12
D13S317: 8,12
D16S539: 11,13
D5S818: 10,13
D7S820: 12
THO1: 6,9
TPOX: 11
vWA: 16
Cytogenetic Analysis: This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t（4q21q）; der（5）t（2;5） （q11.2;q13）; i（5p）; del（17） （p11.2）; del（10） （q22.1） and four others. Of these, del（17） generally had two copies per cell. Seven or more other marker chromosomes including HSR（11） （p15.1） were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.
Age: 33 years
Gender: female
Ethnicity: Asian
Comments: SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. The cells express the surface glycoproteins carcinoembryonic antigen （CEA） and TAG-72. The cells are L-dopa decarboxylase （DDC） positive. SNU-16 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Medium Renewal: Add fresh medium every 3 to 4 days （depending on cell density）
Preservation: Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 27 hrs
References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397
23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..
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ATCC® Number: CRL-5974™ Price: $425.00
Designations: SNU-16
Depositors: J Park
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, multicell aggregates
Organism: Homo sapiens （human）
Morphology: epithelial
CRL-5974 SNU-16 人胃癌細胞 腹水
Source: Organ: stomach
Disease: gastric carcinoma
Derived from metastatic site: ascites
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1987
Receptors: vasoactive intestinal peptide （VIP）, expressed [23078]
Oncogene: myc +; erb B2 +
Antigen Expression: Blood Type A; Rh +
The cells express the surface glycoproteins carcinoembryonic antigen （CEA） and TAG 72.
DNA Profile （STR）: Amelogenin: X
CSF1PO: 12
D13S317: 8,12
D16S539: 11,13
D5S818: 10,13
D7S820: 12
THO1: 6,9
TPOX: 11
vWA: 16
Cytogenetic Analysis: This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t（4q21q）; der（5）t（2;5） （q11.2;q13）; i（5p）; del（17） （p11.2）; del（10） （q22.1） and four others. Of these, del（17） generally had two copies per cell. Seven or more other marker chromosomes including HSR（11） （p15.1） were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.
Age: 33 years
Gender: female
Ethnicity: Asian
Comments: SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. The cells express the surface glycoproteins carcinoembryonic antigen （CEA） and TAG-72. The cells are L-dopa decarboxylase （DDC） positive. SNU-16 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Medium Renewal: Add fresh medium every 3 to 4 days （depending on cell density）
Preservation: Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 27 hrs
References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397
23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..
Related Links
NCBI Entrez Search
Make a Deposit
Frequently Asked Questions
Material Transfer Agreement
Technical Support
Related Cell Culture Products
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